August 9, 2012 - 1:00pm EDT
Presented by:
Bruce Aronow
PhD - Co-director, Computational Medicine Center, Cincinnati Children’s Hospital Medical Center;
Professor, Biomedical Informatics, Cincinnati Children’s Hospital Medical Center
Phillip Dexheimer,
MS Computer Science - Senior Developer,
Nexgen Sequencing Data Genome Bioinformatics Core
Title: "Building a NexGen Gene Expression Database for the Human Progenitor Cell Biology Consortium"
View: (Archived Webinar Recording)
Abstract:
A key goal for the NHLBI Progenitor Cell Biology Consortium is to characterize a wide variety of stem cell lineages using integrative omics technologies including RNAseq, miRseq, DNA methylation, CNV genomic integrity and cell surface marker expression as a function of cell of origin, method of stem cell generation, and the ability to undergo subsequent lineage differentiation. In order to generate a resource that can have the maximum possible impact for the stem cell community, the data from this characterization must be easily accessible in both a high-level “key findings” form and a low-level form that permits bioinformaticians to perform their own analysis. In addition, we would like to take advantage of the novel capabilities of next-generation sequencing, including novel transcript discovery and isoform quantification, to comprehensively determine the molecular basis of progenitor cell biology.
Speaker Bios:
Dr. Aronow received his BS in chemistry from Stanford University in 1976 and was also a member of the Honors in Physics undergraduate program. He completed his PhD in Biochemistry and Genetics at the University of Kentucky. He is a Professor at the University of Cincinnati and is a member of the Cincinnati Children’s Hospital Research Foundation in the Divisions of Biomedical Informatics, Developmental Biology, and Departments of Pediatrics and Computer Science at the University of Cincinnati. Dr. Aronow is a passionate collaborator with people from multiple technical, research and clinical disciplines. His goal is to accelerate research using computational bioinformatics and integrative systems biology analysis approaches across the entire biomedical sciences spectrum. Dr. Aronow has played a variety of roles in multi-investigator genome/phenome-centered projects that share in seeking to understand the molecular, cellular and physiologic basis of normal and abnormal systems functions and to translate findings and tests between model systems and human diseases.
Mr. Dexheimer received his BS in Computer Science from the University of Central Florida in 2000, followed by an MS from the same department in 2003. Since 2004, he has been a developer/analyst in high-throughput genomics cores, moving with the technology from microarrays to high-throughput sequencing first for 5 years at the Vanderbilt Microarray Shared Resource and then at the Hudson Alpha Research Institute for 2 years. He is currently a Senior Systems Programmer in the Divisions of Biomedical Informatics and Human Genetics at the Cincinnati Children's Hospital Research Foundation, specializing in data quality control and automating the primary analysis of high-throughput sequencing experiments.